Primer
Part:BBa_K4998002:Design
Designed by: Markos Mathioudakis Group: iGEM23_thessaloniki (2023-10-06)
Laccase gene left primer
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 9
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 14
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 9
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 9
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
From the software's proposed primers we choce the one with the longest product size so that we can observe a band in the agarose GEL after the PCR.
Source
We designed this part using the Primer3 software.